Analysis Note

ControlHeLa, NIH/3T3 and NTERA-2 cell lysates

Application

Anti-WIF1 Antibody, clone 1G5 is a highly specific mouse monoclonal antibody, that targets Wnt inhibitory factor 1 & has been tested in western blotting, IHC & Immunofluorescence.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in HeLa cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in human ovarian tumor and lung cancer tissues.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

WIF1, also known as Wnt inhibitory factor 1 or WIF-1, is a secreted protein that binds to Wnt proteins and inhibits their activity. WNT proteins have been shown to act as an extracellular signaling molecules and may be involved in the control of embryonic development. WIf1 contains an N-terminal WIF domain and 5 epidermal growth factor (EGF)-like domains. It has been implied in recent sutyes that WIF1 expression is downregulated in many types of cancers.

Immunogen

Purified recombinant fragment of human WIF1 expressed in E. Coli.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HeLa, NIH/3T3 and NTERA-2 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected WIF1 in HeLa, NIH/3T3 and NTERA-2 cell lysates.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~43 kDa observed. Uncharacterized bands may appear in some lysate(s).